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标题: 两种十大功劳叶中生物碱成分的分析与比较
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作者: 刘偲翔[1] 刘布鸣[2] 何开家[2] 陈明生[2] 林霄[2]
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关键字词: 十大功劳叶 含量测定 药根碱 巴马汀 小檗碱 反相高效液相色谱法
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摘要: 目的建立长柱十大功劳叶和阔叶十大功劳叶中药根碱、巴马汀、小檗碱的含量测定方法,比较两者的生物碱成分含量差异。方法采用反相高效液相色谱外标法同时测定。色谱柱为大连依利特Sino Chrom ODS—BP柱(250mm×4.6mm,5μm),流动相为乙腈-0.2%磷酸(23:77),流速为1.0mL/min,检测波长为265nm,进样量为10μL。结果上述色谱条件下,盐酸药根碱、盐酸巴马汀、盐酸小檗碱可以较好地分离,线性范围分别为0.001444—0.07076μg(r=0.9999),0.0005152~0
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