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标题: 高效液相色谱法测定复方葛酮颗粒中葛根素含量
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作者: 杨冬梅[1] 杨世友[2]
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关键字词: 复方葛酮颗粒 葛根素 高效液相色谱法 含量测定
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摘要: 目的建立测定复方葛酮颗粒中葛根素含量的高效液相色谱(HPLC)法。方法以Waters C18柱(250mm×4.6mm,5μm)为色谱柱,流速1.0mL/min,检测波长250nm,甲醇-水-醋酸(25:75:1)为流动相。结果葛根素进样量的线性范围为0.048~1.92μg(r=0.9999,n=6)。平均回收率为99.12%,RSD为1.62%(n=6)。结论HPLC法简便、准确,重现性好,可用于复方葛酮颗粒的质量控制。[著者文摘]
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