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标题: 高效液相色谱法同时测定复方地芬诺酯片中两组分含量
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作者: 何建锋[1] 郭瑞锋[2] 郄冰冰[2]
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关键字词: 高效液相色谱法 复方地芬诺酯片 盐酸地芬诺酯 硫酸阿托品 含量测定
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摘要: 目的建立同时测定复方地芬诺酯片中盐酸地芬诺酯和硫酸阿托品含量的高效液相色谱(HPLC)法。方法色谱柱为岛津VP—ODS柱(150mm×4.6mm,5μm),流动相为0.5%十二烷基硫酸钠溶液(用70%磷酸调节pH至2.7)-乙腈(35:65),检测波长210nm,柱温30℃,流速1.0mL/min。结果盐酸地芬诺酯和硫酸阿托品质量浓度线性范围分别为10.12~505.6μg/mL(r=0.9999)和2.36—118.0μg/mL(r=0.9999),平均回收率分别为100.17%(RSD=1.22%)和
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