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标题: 反相高效液相色谱法测定注射用头孢美唑钠的含量
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作者: 边原 何林 夏祺悦
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关键字词: 反相高效液相色谱法 头孢美唑钠 稳定性
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摘要: 目的建立测定注射用头孢美唑钠含量的反相高效液相色谱法,并考察其常用试验条件下的稳定性。方法采用生理盐水溶解,流动相进行稀释。色谱柱为C18柱(150mm×4.6mm,5μm),流动相为磷酸二氢铵水溶液-甲醇-四氢呋喃-10%四丁基氢氧化铵(700:280:25:12.8),流速为1 mL/min,检测波长为272nm,柱温为30℃。结果注射用头孢美唑钠的质量浓度线性范围是1-100μg/mL(r=0.9999),方法平均回收率为99.20%,日内、日间精密度RSD均小于1%(n=5)。头孢关唑对照品溶液5
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